Difference between revisions of "Part:BBa K4586008"

Revision as of 12:59, 22 September 2023

MS2

Part Description

This part is a highly specific RNA hairpin loop structure that has high affinity for MCP to navigate the MCP-ADAR2 to the sequence of interest by flanking the sequence with two MS2 hairpin structures.

Usage

This part is used in our design to direct the activity of MCP-ADAR2 to the sensor sequence that is flanked by two MS2 hairpin structures by the RNA binding domain MS2 coat protein (MCP). Therefore, the sensitivity of this system is markedly enhanced as shown in figure 1.

Figure 1: This figure illustrate the activity of our DART V ADAR tissue specific switch that is designed to be in the on state after recognition of the autoreactive B-cells,this recognition based on mismatched base editing in the level of transcribed RNA that is mediated through ADAR enzyme activity.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ==Usage==
-This part is used in our design to direct the activity of MCP-ADAR2  to the sensor sequence that is flanked by two MS2 hairpin structures by the RNA binding domain MS2 coat protein (MCP).  Therefore, the sensitivity of this system is markedly enhanced.
+This part is used in our design to direct the activity of MCP-ADAR2  to the sensor sequence that is flanked by two MS2 hairpin structures by the RNA binding domain MS2 coat protein (MCP).  Therefore, the sensitivity of this system is markedly enhanced as shown in figure 1.
+<html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              	max-width:850px;
+							width:100%;
+							height:auto;
+								position: relative;
+							top: 50%;
+							left: 45%;
+							transform: translate( -50%);
+							padding-bottom:25px;
+							padding-top:25px;
+							"src="https://static.igem.wiki/teams/4586/wiki/parts/ms2-sensor-mcp-adar-7.png
+">
+<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
+lang=EN style='font-size:11.0pt;line-height:115%'>Figure 1: This figure illustrate the activity of our DART V ADAR tissue specific switch that is designed to be in the on state after recognition of the autoreactive B-cells,this recognition based on mismatched base editing in the level of transcribed RNA that is mediated through ADAR enzyme activity.   </span></p></div></html>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===