Difference between revisions of "Part:BBa K4806004"

Revision as of 11:46, 20 September 2023

CYP9Q3 gene for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the coding sequence of CYP9Q3 (B3-B4). This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. In combination with a promoter like AβSAP(i) (BBa_K4806013) and a terminator like tRPL23 (BBa_K3002006)^*, this level 0 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015). To detect the target protein a tag like HA-tag (BBa_K3002017)^* is recommended.

Constructs

Here are the links to the built constructs:

• 1. CYP9Q3 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick) (BBa_K4806222)
• 2. CYP9Q3 gene with mStop for Chlamydomonas reinhardtii (Phytobrick) (BBa_K4806223)
• 3. CYP9Q3 gene with mNeonGreen for Chlamydomonas reinhardtii (Phytobrick) (BBa_K4806224)

These constructs were transformed into Chlamydomonas reinhardtii. Besides the CYP9Q3 coding sequence the constructs contain the AβSAP(i)-promotor (BBa_K4806013), either the HA-tag (BBa_K3002017)^* or mNeonGreen (BBa_K4806006) for detection or mStop (BBa_K4806009) and the tRPL23-terminator (BBa_K3002006)^*. The resistance cassette for spectinomycin is already built in the level 2 vector pMBS807 we are using. The usage of this vector allows the direct assembly of level 0 parts to level 2 constructs, facilitating the cloning time (Niemeyer & Schroda, 2022).

Sequence and Features

Results