Difference between revisions of "Part:BBa K4586014"
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We implemented this part in our design to form our loading system for the therapeutic agent to be loaded into exosomes. This loading system is composed of three elements: First, CD63, which is a transmembrane protein naturally present and highly expressed on the external surface of the exosome membrane, second L7Ae, which is a RNA-binding protein conjugated to the internal domain of the CD63 protein, in order to bind to the third element of the loading system present in the 3` end of our cargo, which is the C\D box or kink turn.
 
We implemented this part in our design to form our loading system for the therapeutic agent to be loaded into exosomes. This loading system is composed of three elements: First, CD63, which is a transmembrane protein naturally present and highly expressed on the external surface of the exosome membrane, second L7Ae, which is a RNA-binding protein conjugated to the internal domain of the CD63 protein, in order to bind to the third element of the loading system present in the 3` end of our cargo, which is the C\D box or kink turn.
  
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https://static.igem.wiki/teams/4586/wiki/parts/14-15-26-part-2.png
  
  

Revision as of 08:31, 21 September 2023


CD63

Part Description

CD63 is a member of tetraspanin superfamily which is a hall mark natural protein highly expressed on the external surface of the exosomes.

Usage

We implemented this part in our design to form our loading system for the therapeutic agent to be loaded into exosomes. This loading system is composed of three elements: First, CD63, which is a transmembrane protein naturally present and highly expressed on the external surface of the exosome membrane, second L7Ae, which is a RNA-binding protein conjugated to the internal domain of the CD63 protein, in order to bind to the third element of the loading system present in the 3` end of our cargo, which is the C\D box or kink turn.

14-15-26-part-2.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]