Difference between revisions of "Part:BBa K191007:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K191007 short</partinfo>
 
<partinfo>BBa_K191007 short</partinfo>
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===Design Notes===
 
===Design Notes===
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2 SpeI enzyme restriction sites on Trp operon sequence.  
+
*Notice: 2 SpeI enzyme restriction sites on Trp promoter sequence.  
  
 +
*Trp promoter sequence is ordered as primer and added to part <partinfo>BBa_K191004</partinfo> and <partinfo>BBa_K191005</partinfo> by PCR.
  
  
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Sequence of Trp operon was found at : http://biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU00067
 
Sequence of Trp operon was found at : http://biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU00067
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Part was added to the system as the upstream sequence of the forward primer in a PCR.
 
  
 
===References===
 
===References===

Latest revision as of 13:53, 21 October 2009

Trp promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Notice: 2 SpeI enzyme restriction sites on Trp promoter sequence.


Source

Sequence of Trp operon was found at : http://biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU00067

References