Difference between revisions of "Part:BBa K4719009"

Revision as of 14:27, 17 September 2023

pKARA_RT3 styrene monooxigenase

Introduction

Vilnius Lithuania iGEM 2023 team's goal was to create a universal synthetic biology system in Komagataeibacter xylinus for in vivo bacterial cellulose polymer composition modification. As a second approach, we designed indigo-dyed cellulose that could be used as a green chemistry way to apply cellulose in the textile industry.

Colorful cellulose was made by introducing styrene monooxygenase pKARA_RT3 BBa_K4719018 to K. xylinus. This enzyme can metabolize indigo and its other derivatives into indigo dyes. Bacteria produce cellulose alongside pigments. Since they are not water soluble, the final product retains the color.

Usage and Biology

pKARA_RT3 is styrene monooxygenase. Styrene monooxygenases belong to oxidoreductases, specifically to an enzyme class 1.14.14-, which act on paired donors, with incorporation or reduction of molecular oxygen, which is donated by reduced flavin or flavoprotein. Styrene monooxygenases have been shown to produce indigo - a blue dye (1). Cells containing this enzyme are able to act as biocatalysts and convert indole to indigo. pKARA_RT3 is a basic part in BBa_K4719018, this construct is used for the production of colorful cellulose.

Characterisation

References

1. O’Connor, K.E., Dobson, A.D. and Hartmans, S. (1997) ‘Indigo formation by microorganisms expressing styrene monooxygenase activity’, Applied and Environmental Microbiology, 63(11), pp. 4287–4291. doi:10.1128/aem.63.11.4287-4291.1997.

Sequence and Features