Difference between revisions of "Part:BBa K4656012"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4656012 short</partinfo> | <partinfo>BBa_K4656012 short</partinfo> | ||
| − | MleS placed downstream of the T7 promotor is a malate-binding transcriptional activator belonging to l. lactis, which has been characterized by iGEM22_GEMS_Taiwan in 2022. | + | MleS placed downstream of the T7 promotor is a malate-binding transcriptional activator belonging to l. lactis, which has been characterized by iGEM22_GEMS_Taiwan in 2022. P_MleS is taken from the putative promoter region of mleS by iGEM22_GEMS_Taiwan, this is, it is a binding site of MleS. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | In our circuit, the Mles can tightly bind to P_MleS in the presence of malate, which can change the conformation of Mles, thereby activating P_MleS and the expression of downstream mazF. In this design, the addition of malate can inhibit the growth of engineered E. coli and induce apoptosis. | ||
===Experimental results=== | ===Experimental results=== | ||
Revision as of 11:25, 17 September 2023
pT7-mleR-p_mleS-mazF
MleS placed downstream of the T7 promotor is a malate-binding transcriptional activator belonging to l. lactis, which has been characterized by iGEM22_GEMS_Taiwan in 2022. P_MleS is taken from the putative promoter region of mleS by iGEM22_GEMS_Taiwan, this is, it is a binding site of MleS.
Usage and Biology
In our circuit, the Mles can tightly bind to P_MleS in the presence of malate, which can change the conformation of Mles, thereby activating P_MleS and the expression of downstream mazF. In this design, the addition of malate can inhibit the growth of engineered E. coli and induce apoptosis.
Experimental results
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 68
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]