Difference between revisions of "Part:BBa K4656011"
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<partinfo>BBa_K4656011 short</partinfo> | <partinfo>BBa_K4656011 short</partinfo> | ||
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| + | L-rhamnose-inducible promoter is capable of high-level recombinant protein expression in the presence of L-rhamnose， it is also tightly regulated in the absence of L-rhamnose by the addition of D-glucose, which has been well characterized by Paris_Bettencour in 2012. Tetracycline repressor (TetR) is an effector-regulated DNA-binding protein that binds tightly to its palindromic tetO operator DNA in the absence of an effector, tet or its analogs, thereby blocking the transcription of any downstream genes. TetO, placed downstream of TetR, as mentioned before, can be repressed by TetR. Escherichia coli MazF is a toxin protein that cleaves RNA at ACA sequences, thus causing growth inhibition and block protein synthesis[1]. | ||
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===Experimental results=== | ===Experimental results=== | ||
| + | ===Reference=== | ||
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| + | [1] Zhang, Y., Zhang, J., Hoeflich, K. P., Ikura, M., Qing, G., & Inouye, M. (2003). MazF cleaves cellular mRNAs specifically at ACA to block protein synthesis in Escherichia coli. Molecular cell, 12(4), 913–923. https://doi.org/10.1016/s1097-2765(03)00402-7 | ||
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Revision as of 09:50, 17 September 2023
pRha-tetR-tetO-mazF
L-rhamnose-inducible promoter is capable of high-level recombinant protein expression in the presence of L-rhamnose， it is also tightly regulated in the absence of L-rhamnose by the addition of D-glucose, which has been well characterized by Paris_Bettencour in 2012. Tetracycline repressor (TetR) is an effector-regulated DNA-binding protein that binds tightly to its palindromic tetO operator DNA in the absence of an effector, tet or its analogs, thereby blocking the transcription of any downstream genes. TetO, placed downstream of TetR, as mentioned before, can be repressed by TetR. Escherichia coli MazF is a toxin protein that cleaves RNA at ACA sequences, thus causing growth inhibition and block protein synthesis[1].
Usage and Biology
Experimental results
Reference
[1] Zhang, Y., Zhang, J., Hoeflich, K. P., Ikura, M., Qing, G., & Inouye, M. (2003). MazF cleaves cellular mRNAs specifically at ACA to block protein synthesis in Escherichia coli. Molecular cell, 12(4), 913–923. https://doi.org/10.1016/s1097-2765(03)00402-7
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]