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| Line 11: |
Line 11: |
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| | synthesized by purimex | | synthesized by purimex |
| − | LOCUS His+FluA+Split+F 3616 bp DNA circular SYN 08-OCT-2009
| + | |
| − | DEFINITION Ligation of His+FluA+Split+FokI into pMA2_NgoMIV_SpeI
| + | [https://static.igem.org/mediawiki/2009/d/d8/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.txt Tata] |
| − | ACCESSION His+FluA+Split+F
| + | |
| − | KEYWORDS .
| + | |
| − | SOURCE Unknown.
| + | |
| − | ORGANISM Unknown
| + | |
| − | Unclassified.
| + | |
| − | REFERENCE 1 (bases 1 to 3616)
| + | |
| − | AUTHORS Self
| + | |
| − | JOURNAL Unpublished.
| + | |
| − | COMMENT SECID/File created by SciEd Central, Scientific & Educational Software
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| − | COMMENT SECNOTES|Vector molecule: pMA2_NgoMIV_SpeI
| + | |
| − | Fragment ends: SpeI and NgoMIV
| + | |
| − | Fragment size: 2412
| + | |
| − | Insert molecule: His+FluA+Split+FokI
| + | |
| − | Fragment ends: NgoMIV and SpeI
| + | |
| − | Fragment size: 1204
| + | |
| − | FEATURES Location/Qualifiers
| + | |
| − | CDS 129..989
| + | |
| − | /gene="AmpR"
| + | |
| − | /SECDrawAs="Gene"
| + | |
| − | CDS 2008..3213
| + | |
| − | /gene="ORF-1"
| + | |
| − | /SECDrawAs="Gene"
| + | |
| − | CDS 2017..2034
| + | |
| − | /gene="His Tag"
| + | |
| − | /SECDrawAs="Gene"
| + | |
| − | CDS 2041..2562
| + | |
| − | /gene="FluA"
| + | |
| − | /SECDrawAs="Gene"
| + | |
| − | CDS 2569..2619
| + | |
| − | /gene="Split-Linker"
| + | |
| − | /SECDrawAs="Gene"
| + | |
| − | CDS 2623..3212
| + | |
| − | /gene="'Fok inaktiv'"
| + | |
| − | /SECDrawAs="Gene"
| + | |
| − | ORIGIN
| + | |
| − | 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
| + | |
| − | 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
| + | |
| − | 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
| + | |
| − | 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
| + | |
| − | 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
| + | |
| − | 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
| + | |
| − | 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
| + | |
| − | 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
| + | |
| − | 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
| + | |
| − | 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
| + | |
| − | 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
| + | |
| − | 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
| + | |
| − | 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
| + | |
| − | 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
| + | |
| − | 841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
| + | |
| − | 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
| + | |
| − | 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
| + | |
| − | 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
| + | |
| − | 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
| + | |
| − | 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
| + | |
| − | 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
| + | |
| − | 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
| + | |
| − | 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
| + | |
| − | 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
| + | |
| − | 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
| + | |
| − | 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
| + | |
| − | 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
| + | |
| − | 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
| + | |
| − | 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
| + | |
| − | 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
| + | |
| − | 1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga
| + | |
| − | 1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga
| + | |
| − | 1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag
| + | |
| − | 1981 aggtaccgaa ttcgcggccg cttctagatg gccggccatc atcatcatca tcataccggc
| + | |
| − | 2041 gacgtgtacc acgacggcgc ctgccccgaa gtgaagcccg tggacaactt cgactggtcc
| + | |
| − | 2101 cagtaccacg gcaagtggtg ggaggtggcc aagtacccca gccccaacgg caagtatggc
| + | |
| − | 2161 aagtgcggct gggccgagta cacccccgag ggcaagagcg tgaaggtgtc cagatacgac
| + | |
| − | 2221 gtgatccacg gcaaagaata cttcatggaa ggcaccgcct accccgtggg cgacagcaag
| + | |
| − | 2281 atcggcaaga tctaccacag ccggaccgtg ggcggctaca ccagaaagac cgtgttcaac
| + | |
| − | 2341 gtgctgtcca ccgacaacaa gaactacatc atcggctaca gctgccgcta cgacgaggac
| + | |
| − | 2401 aagaagggcc actgggacca cgtgtgggtg ctgtcccggt ccatggtgct gaccggcgag
| + | |
| − | 2461 gccaagaccg ccgtggagaa ctacctgatc ggcagccccg tggtggacag ccagaaactg
| + | |
| − | 2521 gtgtacagcg acttcagcga ggccgcctgc aaagtgaaca acaccggccg accagcctgt
| + | |
| − | 2581 aagattccaa atgacctgaa gcagaaagtt atgaatcaca ccggcaaatc tgaactggag
| + | |
| − | 2641 gagaaaaaat ccgagctgcg ccacaaactg aaatatgtgc ctcacgagta tatcgaactg
| + | |
| − | 2701 atcgagatcg cccgtaatag tacccaagac cgtatcctgg aaatgaaagt gatggagttc
| + | |
| − | 2761 ttcatgaaag tctatggcta tcgtggcaaa catctgggtg gtagccgtaa accagcaggt
| + | |
| − | 2821 gccatttata ccgttggttc cccgatcgat tatggcgtta tcgtggccac aaaagcgtat
| + | |
| − | 2881 tctggcggtt ataatctgcc gattggtcag gctgatgaga tggaacgtta tgtggaagag
| + | |
| − | 2941 aatcagaccc gtaacaaaca tctgaacccg aacgaatggt ggaaagtgta tccgtcaagt
| + | |
| − | 3001 gtcaccgagt tcaaatttct gttcgtgagc ggccacttta aaggcaacta taaagcccag
| + | |
| − | 3061 ctgactcgtc tgaaccatat caccaatagc aatggggcag tgctgagtgt tgaggaactg
| + | |
| − | 3121 ctgatcggtg gagaaatgat caaagcaggc accctgactc tggaagaagt tcgccgtaaa
| + | |
| − | 3181 ttcaacaatg gcgagatcaa ttttaccggt taatactagt agcggccgct gcagagctca
| + | |
| − | 3241 tggcgcgcct aggccttgac ggccttccgc caattcgccc tatagtgagt cgtattacgt
| + | |
| − | 3301 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
| + | |
| − | 3361 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
| + | |
| − | 3421 ccgaaacgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggagcg ccctgtagcg
| + | |
| − | 3481 gccactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
| + | |
| − | 3541 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
| + | |
| − | 3601 aacgcttaca atttag
| + | |
| − | //
| + | |
| | | | |
| | ===References=== | | ===References=== |
