Difference between revisions of "Part:BBa K4727106"

Latest revision as of 19:47, 27 September 2023

sgDNA for Orf3/CraA 110 for A. baumannii

This guide DNA has been designed to work with dCas9 to target AMR related genes in Acinetobacter baumannii . The guide binds 110bp downstream the TSS

CraA (which stands for chloramphenicol resistance Acinetobacter, originally named Orf3) is a multidrug efflux transport protein homologous to the MdfA efflux pump of Escherichia coli. The system has been found in all 82 A. baumannii strains tested in [1] and is believed to contribute to intrinsic resistance to chloramphenicol [1]. The silencing of this gene significantly decreases the MIC (Minimum Inhibitory Concentration) for the antibiotic chloramphenicol [2].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

[1] Coyne, Sébastien, Patrice Courvalin, e Bruno Périchon. «Efflux-Mediated Antibiotic Resistance in Acinetobacter Spp». Antimicrobial Agents and Chemotherapy 55, fasc. 3 (marzo 2011): 947–53. https://doi.org/10.1128/AAC.01388-10.

[2] Roca, I., S. Marti, P. Espinal, P. Martínez, I. Gibert, e J. Vila. «CraA, a Major Facilitator Superfamily Efflux Pump Associated with Chloramphenicol Resistance in Acinetobacter Baumannii». Antimicrobial Agents and Chemotherapy 53, fasc. 9 (settembre 2009): 4013–14. https://doi.org/10.1128/AAC.00584-09.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4727106 short</partinfo>
-This guide DNA has been designed to work with dCas9 to target AMR related genes in
+This guide DNA has been designed to work with dCas9 to target AMR related genes in <i>Acinetobacter baumannii </i>. The guide binds 110bp downstream the TSS
+CraA (which stands for chloramphenicol resistance <i>Acinetobacter</i>, originally named Orf3) is a multidrug efflux transport protein homologous to the MdfA efflux pump of <i>Escherichia coli</i>. The system has been found in all 82 <i>A. baumannii</i> strains tested in [1] and is believed to contribute to intrinsic resistance to chloramphenicol [1].
+The silencing of this gene significantly decreases the MIC (Minimum Inhibitory Concentration) for the antibiotic chloramphenicol [2].
 <!-- Add more about the biology of this part here
@@ Line 12: / Line 15: @@
 <partinfo>BBa_K4727106 SequenceAndFeatures</partinfo>
+===References===
+[1] Coyne, Sébastien, Patrice Courvalin, e Bruno Périchon. «Efflux-Mediated Antibiotic Resistance in Acinetobacter Spp». Antimicrobial Agents and Chemotherapy 55, fasc. 3 (marzo 2011): 947–53. https://doi.org/10.1128/AAC.01388-10.
+[2] Roca, I., S. Marti, P. Espinal, P. Martínez, I. Gibert, e J. Vila. «CraA, a Major Facilitator Superfamily Efflux Pump Associated with Chloramphenicol Resistance in Acinetobacter Baumannii». Antimicrobial Agents and Chemotherapy 53, fasc. 9 (settembre 2009): 4013–14. https://doi.org/10.1128/AAC.00584-09.
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