Difference between revisions of "Part:BBa K4768008"
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<h2>Introduction</h2>
 
<h2>Introduction</h2>
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            <figcaption class="normal"><span class="titre-image"><i><b>Figure 1: NT7-ZipA-Pertuzumab structure.</b></i></span></figcaption>
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<p>The CALIPSO part BBa_K4768008 is composed of the N-terminal subunit of the T7 RNA polymerase (residues 1 to 180) fused to the anti-HER2 antibody Pertuzumab through an optimized linker sequence containing a transmembrane domain. This gene is under transcriptional control of an SP6 promoter and T7 terminator. </p>
  
 
<h2>Construction</h2>
 
<h2>Construction</h2>

Revision as of 12:31, 5 October 2023


Split T7 RNA polymerase (Nterm) conjugated to Pertuzumab with a transmembrane linker

Part for Expression of the T7 RNA polymerase (Nterm) conjugated to Pertuzumab with a transmembrane linker ZipA

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 987
    Illegal XbaI site found at 40
    Illegal SpeI site found at 1147
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 987
    Illegal SpeI site found at 1147
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 987
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 987
    Illegal XbaI site found at 40
    Illegal SpeI site found at 1147
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 987
    Illegal XbaI site found at 40
    Illegal SpeI site found at 1147
    Illegal AgeI site found at 1200
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 21
    Illegal SapI.rc site found at 1836


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Introduction

Figure 1: NT7-ZipA-Pertuzumab structure.

The CALIPSO part BBa_K4768008 is composed of the N-terminal subunit of the T7 RNA polymerase (residues 1 to 180) fused to the anti-HER2 antibody Pertuzumab through an optimized linker sequence containing a transmembrane domain. This gene is under transcriptional control of an SP6 promoter and T7 terminator.

Construction

TXXXXXX.

Molecular Modeling

TXXXXXX.

Characterisation

TXXXXXX.

Conclusion and Perspectives

TXXXXXX.

References

  1. article 1 xxxxxxxx
  2. article 2 xxxxxxx
    3.