Difference between revisions of "Part:BBa K4606004"

 
 
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__NOTOC__
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<partinfo>BBa_K4606004 short</partinfo>
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==Description==
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    <p>For detailed design information, you can look up the registry of <a href="https://parts.igem.org/Part:BBa_K4606003">BBa_K4606003</a>, the device for gene segment of SMAD4.</p>
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    <p>According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.</p>
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    <div style="margin:auto; width: 400px">   
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        <img src="https://static.igem.wiki/teams/4606/wiki/part-4-smad4-mut.jpg" width="400px">
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    </div>
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    <div style="margin:auto; width:400px;">
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        <p>Figure3 (Part4). Acquisition of SMAD4-MUT gene segments by PCR.
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            <br>1#: DNA marker. 2#: Segments of target gene.
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            </p>
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    </div>
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</html>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4606004 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4606004 parameters</partinfo>
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Latest revision as of 13:12, 18 September 2023


SMAD4(mutant type)

Description

For detailed design information, you can look up the registry of BBa_K4606003, the device for gene segment of SMAD4.

According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.

Figure3 (Part4). Acquisition of SMAD4-MUT gene segments by PCR.
1#: DNA marker. 2#: Segments of target gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]