Difference between revisions of "Part:BBa K4806001:Design"

(Replaced content with " __NOTOC__ <partinfo>BBa_K4806001 short</partinfo> <partinfo>BBa_K4806001 SequenceAndFeatures </partinfo> ===Design Notes=== This part has been codon optimized for Chl...")
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<partinfo>BBa_K4806001 short</partinfo>
 
<partinfo>BBa_K4806001 short</partinfo>
  
<partinfo>BBa_K4806001 SequenceAndFeatures ggcctggaggccctggtgcccctggccgtgatcgtggccatcttcctgctgctggtggacctgatgcaccgccgccagcg
+
<partinfo>BBa_K4806001 SequenceAndFeatures </partinfo>
ctgggcTgcTcgctacagcccTggTcccctgccTctgcccgGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGC
+
TTGCAGATTTGACTTGCAACGCCCGCATTGTGTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAA
+
CCCTGCGTCGCCGTTTCCATTTGCAGgcctgggcaacctgctgcacgtggacttccagaacaccccctactgcttcgacc
+
agctgcgccgccgcttcggTgacgtgttcagcctgcagctggcTtggaccccTgtggtggtgctgaacggcctggcTgcT
+
gtgcgcgaggccctggtgacccacggcgaggacaccgccgaccgcccTccTgtgcccatcacccagatcctgggTttcgg
+
TccTcgcagccagggcgtgttcctggcccgctacggTccTgcTtggcgcgagcagcgccgcttcagcgtgagcaccctgc
+
gcaacctgggcctgggcaagaagagcctggagcagtgggtgaccgaggaggccgcctgcctgtgcgccgccttcgccaac
+
cacagcgGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGTGTC
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GACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGgccgcccc
+
ttccgcccTaacggTctgctggacaaggccgtgagcaacgtgatcgccagcctgacctgcggccgccgcttcgagtacga
+
cgacccccgcttcctgcgcctgctggacctggcccaggagggcctgaaggaggagagcggcttcctgcgcgaggtgctga
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acgccgtgcccgtgctgctgcacatccccgccctggccggcaaggtgctgcgcttccagaaggccttcctgacccagctg
+
gacgagctgctgaccgagcaccgcatgacctgggacccTgcTcagccTccTcgcgacctgaccgaggccttcctggcTga
+
gatggagaagGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGT
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GTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGgccaa
+
gggcaaccccgagagcagcttcaacgacgagaacctgcgcatcgtggtggccgacctgttcagcgccggcatggtgacca
+
ccagcaccaccctggcctggggcctgctgctgatgatcctgcaccccgacgtgcagcgccgcgtgcagcaggagatcgac
+
gacgtgatcggccaggtgcgccgccccgagatgggcgaccaggcccacatgccctacaccaccgccgtgatccacgaggt
+
gcagcgcttcggcgacatcgtgcccctgggcgtgacccacatgaccagccgcgacatcgaggtgcagggcttccgcatcc
+
ccaagggcaccaccctgatcaccaacctgagcagcgtgctgaaggacgagGTGAGCTTGCGGGGTTGCGAGCAACACTCC
+
AGCAACGAACAGTGCCCAAGTCAGGAATCTGCAGTCAGCCTGGGCTTTCGGCGGCTTTTTCTTGGGCAAACAGCTTGCAC
+
TCATGCCAGCGCGGCTTGTCCAGCCTCACTTGAGCTTTCCAGCTGCTACCAGCCGGGCTATACGACAGCGACAGAGCCAT
+
AGCGTGGAATCACTTATTTGGGTTGCCGAAGTAGCGGTCGGAGCGTGAGTTCTTGGTCAAGCCGCCCCTTATCCGGTTCC
+
TGTCCGTGTCTTTGTCCCTCGTTCACCCTTCGCGGCACCCTTCATCCCCTTGCTTGCAGgccgtgtgggagaagcccttc
+
cgcttccacccTgagcacttcctggacgcTcagggTcacttcgtgaagccTgaggccttcctgcccttcagcgcTggTcg
+
ccgcgcctgcctgggcgagcccctggcccgcatggagctgttcctgttcttcaccagcctgctgcagcacttcagcttca
+
gcgtggcTgcTggTcagccTcgccccagccacagccgcgtggtgagcttcctggtgaccccTagcccctacgagctgtgc
+
gccgtgccccgcgg
+
</partinfo>
+
  
ggcctggaggccctggtgcccctggccgtgatcgtggccatcttcctgctgctggtggacctgatgcaccgccgccagcg
 
ctgggcTgcTcgctacagcccTggTcccctgccTctgcccgGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGC
 
TTGCAGATTTGACTTGCAACGCCCGCATTGTGTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAA
 
CCCTGCGTCGCCGTTTCCATTTGCAGgcctgggcaacctgctgcacgtggacttccagaacaccccctactgcttcgacc
 
agctgcgccgccgcttcggTgacgtgttcagcctgcagctggcTtggaccccTgtggtggtgctgaacggcctggcTgcT
 
gtgcgcgaggccctggtgacccacggcgaggacaccgccgaccgcccTccTgtgcccatcacccagatcctgggTttcgg
 
TccTcgcagccagggcgtgttcctggcccgctacggTccTgcTtggcgcgagcagcgccgcttcagcgtgagcaccctgc
 
gcaacctgggcctgggcaagaagagcctggagcagtgggtgaccgaggaggccgcctgcctgtgcgccgccttcgccaac
 
cacagcgGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGTGTC
 
GACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGgccgcccc
 
ttccgcccTaacggTctgctggacaaggccgtgagcaacgtgatcgccagcctgacctgcggccgccgcttcgagtacga
 
cgacccccgcttcctgcgcctgctggacctggcccaggagggcctgaaggaggagagcggcttcctgcgcgaggtgctga
 
acgccgtgcccgtgctgctgcacatccccgccctggccggcaaggtgctgcgcttccagaaggccttcctgacccagctg
 
gacgagctgctgaccgagcaccgcatgacctgggacccTgcTcagccTccTcgcgacctgaccgaggccttcctggcTga
 
gatggagaagGTGAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGT
 
GTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGgccaa
 
gggcaaccccgagagcagcttcaacgacgagaacctgcgcatcgtggtggccgacctgttcagcgccggcatggtgacca
 
ccagcaccaccctggcctggggcctgctgctgatgatcctgcaccccgacgtgcagcgccgcgtgcagcaggagatcgac
 
gacgtgatcggccaggtgcgccgccccgagatgggcgaccaggcccacatgccctacaccaccgccgtgatccacgaggt
 
gcagcgcttcggcgacatcgtgcccctgggcgtgacccacatgaccagccgcgacatcgaggtgcagggcttccgcatcc
 
ccaagggcaccaccctgatcaccaacctgagcagcgtgctgaaggacgagGTGAGCTTGCGGGGTTGCGAGCAACACTCC
 
AGCAACGAACAGTGCCCAAGTCAGGAATCTGCAGTCAGCCTGGGCTTTCGGCGGCTTTTTCTTGGGCAAACAGCTTGCAC
 
TCATGCCAGCGCGGCTTGTCCAGCCTCACTTGAGCTTTCCAGCTGCTACCAGCCGGGCTATACGACAGCGACAGAGCCAT
 
AGCGTGGAATCACTTATTTGGGTTGCCGAAGTAGCGGTCGGAGCGTGAGTTCTTGGTCAAGCCGCCCCTTATCCGGTTCC
 
TGTCCGTGTCTTTGTCCCTCGTTCACCCTTCGCGGCACCCTTCATCCCCTTGCTTGCAGgccgtgtgggagaagcccttc
 
cgcttccacccTgagcacttcctggacgcTcagggTcacttcgtgaagccTgaggccttcctgcccttcagcgcTggTcg
 
ccgcgcctgcctgggcgagcccctggcccgcatggagctgttcctgttcttcaccagcctgctgcagcacttcagcttca
 
gcgtggcTgcTggTcagccTcgccccagccacagccgcgtggtgagcttcctggtgaccccTagcccctacgagctgtgc
 
gccgtgccccgcgg
 
  
  

Revision as of 12:27, 13 September 2023


CYP2D6 gene for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
    Illegal NotI site found at 862
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 356
    Illegal PstI site found at 1712
    Illegal PstI site found at 2145
    Illegal NgoMIV site found at 999
    Illegal NgoMIV site found at 1348
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii (https://www.kazusa.or.jp/codon/)
We inserted a few mutations to eliminate the recognition sites for our type IIS restriction enzymes BbsI and BsaI.


Source

https://www.uniprot.org/uniprotkb/P10635/entry

References