Difference between revisions of "Part:BBa K4885989"

 
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This part is responsible for the expression of adhE2 gene with Pthl promotor and Dac gene with Pcatl promotor. adhE2 gene is derived from Clostridium acetobutylicum. Dac gene is derived from Clostridium tyrobutyricum (C. tyrobutyricum) L319. It consists of Pthl sequence (BBa_K3443002), strong ribosomal binding site (RBS) sequence  (BBa_K103015),  adhE2 sequence(BBa_K1462060), Dac gene sequence (BBa_K4885000) and terminator sequence  (BBa_K3585002). adhE2 gene codes for the alcohol/aldehyde bifunctional dehydrogenase. Its role is to convert acyl-CoA to aldehyde then to alcohol in two reductive steps using NADH as cofactor. Dac gene codes a NAD-dependent deacetylase which is an enzyme removing acetyl groups from the N-terminal of protein substrates and weakening the N-terminal acetylation of proteins. The enzyme is responsible for the removal of N-terminal acetylation modification in the protein post-translational modification system.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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==Results==
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===（1）Plasmid construction===
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===（2）Construction of C. tyrobutyricum L319 with pMTL-Pthl-adhE2-Dac===
 
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===Functional Parameters===
 
===Functional Parameters===
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Revision as of 06:34, 30 September 2023


100

This part is responsible for the expression of adhE2 gene with Pthl promotor and Dac gene with Pcatl promotor. adhE2 gene is derived from Clostridium acetobutylicum. Dac gene is derived from Clostridium tyrobutyricum (C. tyrobutyricum) L319. It consists of Pthl sequence (BBa_K3443002), strong ribosomal binding site (RBS) sequence (BBa_K103015), adhE2 sequence(BBa_K1462060), Dac gene sequence (BBa_K4885000) and terminator sequence (BBa_K3585002). adhE2 gene codes for the alcohol/aldehyde bifunctional dehydrogenase. Its role is to convert acyl-CoA to aldehyde then to alcohol in two reductive steps using NADH as cofactor. Dac gene codes a NAD-dependent deacetylase which is an enzyme removing acetyl groups from the N-terminal of protein substrates and weakening the N-terminal acetylation of proteins. The enzyme is responsible for the removal of N-terminal acetylation modification in the protein post-translational modification system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Results

（1）Plasmid construction

（2）Construction of C. tyrobutyricum L319 with pMTL-Pthl-adhE2-Dac