Difference between revisions of "Part:BBa K4656003"
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The Lrp is a gene encoding leucine reactive protein, which is expressed by E. coli itself. It mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons, and Lrp mediates this effect for at least some of these operons. For example, it is a regulator of the branched-chain amino acid transport genes. | The Lrp is a gene encoding leucine reactive protein, which is expressed by E. coli itself. It mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons, and Lrp mediates this effect for at least some of these operons. For example, it is a regulator of the branched-chain amino acid transport genes. | ||
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In our project, Lrp dimerizes with butyrate, binds to pPchA promoter, and further produces pchA. | In our project, Lrp dimerizes with butyrate, binds to pPchA promoter, and further produces pchA. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | LRP, as a component of the natural FIM system of E. coli, is an fimS regulator. Both LRP and IHF(Integrated Host factor) may act to regulate the actual inversion events through an involved DNA bending protein complex. In addition, there is the OFF-to-ON inhibitory protein papB and many other hypothesized and known regulators that directly act on LRP. During the course of the project, the FIM system can be considered. (Additional background on FIM systems can be found in the references [1]) | |
| + | ===Reference=== | ||
| + | [1].Schwan WR. Regulation of fim genes in uropathogenic Escherichia coli. World J Clin Infect Dis 2011; 1(1): 17�25. | ||
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===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 15:40, 7 September 2023
Lrp
The Lrp is a gene encoding leucine reactive protein, which is expressed by E. coli itself. It mediates a global response to leucine. Exogenous leucine affects the expression of a number of different operons, and Lrp mediates this effect for at least some of these operons. For example, it is a regulator of the branched-chain amino acid transport genes. In our project, Lrp dimerizes with butyrate, binds to pPchA promoter, and further produces pchA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 30
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]