Difference between revisions of "Part:BBa K4707000:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
Coding sequence was codon optimized for Chlamydomonas reinhardtii. SapI, BsaI, and BpiI
+
Coding sequence was codon optimized for Chlamydomonas reinhardtii. SapI, BsaI, and BpiI recognition sites were removed where necessary by making silent mutations.
  
  

Latest revision as of 20:26, 3 October 2023


AMO Coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coding sequence was codon optimized for Chlamydomonas reinhardtii. SapI, BsaI, and BpiI recognition sites were removed where necessary by making silent mutations.


Source

Methylomicrobium album BG8 is the source of this gene. We are then placing this gene into Chlamydomonas reinhardtii for bioremediation of a lake.

References