Difference between revisions of "Part:BBa K4707000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Coding sequence was codon optimized for Chlamydomonas reinhardtii. SapI, BsaI, and BpiI | + | Coding sequence was codon optimized for Chlamydomonas reinhardtii. SapI, BsaI, and BpiI recognition sites were removed where necessary by making silent mutations. |
Latest revision as of 20:26, 3 October 2023
AMO Coding sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Coding sequence was codon optimized for Chlamydomonas reinhardtii. SapI, BsaI, and BpiI recognition sites were removed where necessary by making silent mutations.
Source
Methylomicrobium album BG8 is the source of this gene. We are then placing this gene into Chlamydomonas reinhardtii for bioremediation of a lake.