Difference between revisions of "Part:BBa K4719017"
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Polyhydroxybutyrate synthesis operon (phaC, phaA, phaB) assembled into pSEVA331-Bb backbone, to produce PHB along with bacterial cellulose in K. xylinus. This copolymer is an alternative to petroleum-based plastics.
 
Polyhydroxybutyrate synthesis operon (phaC, phaA, phaB) assembled into pSEVA331-Bb backbone, to produce PHB along with bacterial cellulose in K. xylinus. This copolymer is an alternative to petroleum-based plastics.
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===Regulated PHB production in ''K. xylinus''===
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We wanted to regulate the amount of PHB in the bacterial cellulose-polyhydroxy butyrate copolymer, therefore the production of PHB was put under an inducible araC-pBAD promoter. Production of PHB is induced after sufficient amount of bacterial cellulose has grown.
  
 
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<!-- Add more about the biology of this part here

Revision as of 15:54, 17 September 2023


phaCAB operon for polyhydroxybutyrate synthesis in K. xylinus

Polyhydroxybutyrate synthesis operon (phaC, phaA, phaB) assembled into pSEVA331-Bb backbone, to produce PHB along with bacterial cellulose in K. xylinus. This copolymer is an alternative to petroleum-based plastics.

Regulated PHB production in K. xylinus

We wanted to regulate the amount of PHB in the bacterial cellulose-polyhydroxy butyrate copolymer, therefore the production of PHB was put under an inducible araC-pBAD promoter. Production of PHB is induced after sufficient amount of bacterial cellulose has grown.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 37
    Illegal PstI site found at 824
    Illegal PstI site found at 1397
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
    Illegal PstI site found at 824
    Illegal PstI site found at 1397
    Illegal NotI site found at 200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 642
    Illegal BamHI site found at 3039
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 37
    Illegal PstI site found at 824
    Illegal PstI site found at 1397
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 37
    Illegal PstI site found at 824
    Illegal PstI site found at 1397
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 368
    Illegal NgoMIV site found at 602
    Illegal NgoMIV site found at 914
    Illegal NgoMIV site found at 1193
    Illegal NgoMIV site found at 1606
    Illegal NgoMIV site found at 1673
    Illegal AgeI site found at 341
  • 1000
    COMPATIBLE WITH RFC[1000]