Difference between revisions of "Part:BBa K4593001:Design"

 
 
Line 12: Line 12:
  
 
===Source===
 
===Source===
−
 
 
Genome of bacteriophage SPN1S.
 
Genome of bacteriophage SPN1S.
  
 
===References===
 
===References===
 +
[1] Lim, J.-S., Shin, H., Kang, D.-H., & Ryu, S. (2012). Characterization of endolysin from a Salmonella Typhimurium-infecting bacteriophage SPN1S. Research in Microbiology, 163(3), 233–241. https://doi.org/10.1016/j.resmic.2012.01.002

Latest revision as of 21:27, 21 August 2023


Spn1s_LysRZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 391
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 890
    Illegal AgeI site found at 1028
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Biobrick assembly, Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.


Source

Genome of bacteriophage SPN1S.

References

[1] Lim, J.-S., Shin, H., Kang, D.-H., & Ryu, S. (2012). Characterization of endolysin from a Salmonella Typhimurium-infecting bacteriophage SPN1S. Research in Microbiology, 163(3), 233–241. https://doi.org/10.1016/j.resmic.2012.01.002