Difference between revisions of "Part:BBa K4887005:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
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Streptococcus pyogenes.Cas9 is a dual RNA-guided DNA endonuclease enzyme associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) adaptive immune system in Streptococcus pyogenes.
  
 
===References===
 
===References===

Revision as of 09:30, 17 August 2023


Cas9


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 907
    Illegal PstI site found at 2329
    Illegal PstI site found at 2533
    Illegal PstI site found at 2563
    Illegal PstI site found at 3775
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 907
    Illegal PstI site found at 2329
    Illegal PstI site found at 2533
    Illegal PstI site found at 2563
    Illegal PstI site found at 3775
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 368
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 907
    Illegal PstI site found at 2329
    Illegal PstI site found at 2533
    Illegal PstI site found at 2563
    Illegal PstI site found at 3775
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 907
    Illegal PstI site found at 2329
    Illegal PstI site found at 2533
    Illegal PstI site found at 2563
    Illegal PstI site found at 3775
    Illegal NgoMIV site found at 1195
    Illegal NgoMIV site found at 2299
    Illegal NgoMIV site found at 2372
    Illegal NgoMIV site found at 2857
    Illegal NgoMIV site found at 3766
    Illegal NgoMIV site found at 4227
    Illegal NgoMIV site found at 4246
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene encoding protein Cas9, which are usually located near the CRISPR array.

Source

Streptococcus pyogenes.Cas9 is a dual RNA-guided DNA endonuclease enzyme associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) adaptive immune system in Streptococcus pyogenes.

References