Difference between revisions of "Part:BBa K4890014:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Our team aims to detect the amounts of heavy metals by transgenic fruit flies. We plan to insert MTF-1 gene driven by UAS and Hid gene driven by MRE in Drosophila embryos, S2 cells, respectively. These two transgenic Drosophila lines interbreed and the offspring with both MTF-1 and Hid genes cross with Drosophila with genotype GMR-GAL4 or Vg-GAL4 or ptc-GAL4. In this way, we constructed three Drosophila lines with genotype UAS-MTF-1;MRE-Hid/GMR-GAL4 , UAS-MTF-1;MRE-Hid/Vg-GAL4 and UAS-MTF-1;MRE-Hid/ptc-GAL4. All these lines can be used as a visual monitoring system for heavy metals. In response to different heavy metal concentrations in the growing environment, they show observable abnormally small eye/wing sizes in the first generation within 5-10 days.  
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===Source===
 
===Source===
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MRE sequence is based on BBa_K4890002.
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Hsp70 sequence is based on BBa_K4890004.
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Hid gene is based on BBa_K4890003.
  
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===References===
 
===References===

Latest revision as of 10:41, 5 October 2023


MRE-Hsp70-Hid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 360
    Illegal XbaI site found at 1616
    Illegal PstI site found at 222
    Illegal PstI site found at 964
    Illegal PstI site found at 1193
    Illegal PstI site found at 1337
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 360
    Illegal PstI site found at 222
    Illegal PstI site found at 964
    Illegal PstI site found at 1193
    Illegal PstI site found at 1337
    Illegal NotI site found at 378
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 360
    Illegal BglII site found at 372
    Illegal XhoI site found at 487
    Illegal XhoI site found at 1393
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 360
    Illegal XbaI site found at 1616
    Illegal PstI site found at 222
    Illegal PstI site found at 964
    Illegal PstI site found at 1193
    Illegal PstI site found at 1337
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 360
    Illegal XbaI site found at 1616
    Illegal PstI site found at 222
    Illegal PstI site found at 964
    Illegal PstI site found at 1193
    Illegal PstI site found at 1337
    Illegal NgoMIV site found at 1322
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our team aims to detect the amounts of heavy metals by transgenic fruit flies. We plan to insert MTF-1 gene driven by UAS and Hid gene driven by MRE in Drosophila embryos, S2 cells, respectively. These two transgenic Drosophila lines interbreed and the offspring with both MTF-1 and Hid genes cross with Drosophila with genotype GMR-GAL4 or Vg-GAL4 or ptc-GAL4. In this way, we constructed three Drosophila lines with genotype UAS-MTF-1;MRE-Hid/GMR-GAL4 , UAS-MTF-1;MRE-Hid/Vg-GAL4 and UAS-MTF-1;MRE-Hid/ptc-GAL4. All these lines can be used as a visual monitoring system for heavy metals. In response to different heavy metal concentrations in the growing environment, they show observable abnormally small eye/wing sizes in the first generation within 5-10 days.


Source

MRE sequence is based on BBa_K4890002. Hsp70 sequence is based on BBa_K4890004. Hid gene is based on BBa_K4890003.


References