Difference between revisions of "Part:BBa K4890011"
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<partinfo>BBa_K4890011 short</partinfo> | <partinfo>BBa_K4890011 short</partinfo> | ||
− | UAS-Hsp70 | + | This part contains transcription activator and promoter derived from pUAST plasmid. It consists of UAS sequence (BBa_K3281012) and Hsp70 sequence (BBa_K4890004). Upstream activating sequence (UAS) is a cis-acting regulatory sequence. It increases the expression of a neighbouring gene when binds to GAL4. Hsp70 is a promoter that can bind to RNA polymerase and start transcription. |
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Revision as of 10:36, 5 October 2023
UAS-Hsp70
This part contains transcription activator and promoter derived from pUAST plasmid. It consists of UAS sequence (BBa_K3281012) and Hsp70 sequence (BBa_K4890004). Upstream activating sequence (UAS) is a cis-acting regulatory sequence. It increases the expression of a neighbouring gene when binds to GAL4. Hsp70 is a promoter that can bind to RNA polymerase and start transcription.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 226
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 226
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 226
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 226
- 1000COMPATIBLE WITH RFC[1000]