Difference between revisions of "Part:BBa K4790009"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4790009 short</partinfo> | <partinfo>BBa_K4790009 short</partinfo> | ||
| − | The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on | + | The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 213th amino acid from phenylalanine to serine. According to Codon preference in E.coli, AGC has the highest possibility coding serine. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 10:25, 4 October 2023
ASR1-F213S-F
The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 213th amino acid from phenylalanine to serine. According to Codon preference in E.coli, AGC has the highest possibility coding serine.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]