Difference between revisions of "Part:BBa K4765013"
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| − | ==Introduction== | + | ===Introduction=== |
MAA analogues like shinorine or porphyra-334 is transformed from MG by a D-Ala-D-Ala ligaselike enzyme gene MysD<ref>Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. ''The Journal of Organic Chemistry, 86''(16), 11160–11168.</ref>. | MAA analogues like shinorine or porphyra-334 is transformed from MG by a D-Ala-D-Ala ligaselike enzyme gene MysD<ref>Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. ''The Journal of Organic Chemistry, 86''(16), 11160–11168.</ref>. | ||
| − | ==Usage and Biology== | + | ===Usage and Biology=== |
We performed codon optimization on [https://parts.igem.org/Part:BBa_K4273013 BBa_K4273013] (NlmysD) specifically for the ''Escherichia coli'' K12 strain, resulting in the creation of this part. The enzyme MysD catalyzes the fourth reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within ''E. coli''. | We performed codon optimization on [https://parts.igem.org/Part:BBa_K4273013 BBa_K4273013] (NlmysD) specifically for the ''Escherichia coli'' K12 strain, resulting in the creation of this part. The enzyme MysD catalyzes the fourth reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within ''E. coli''. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
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==References== | ==References== | ||
<references /> | <references /> | ||
Revision as of 04:05, 30 September 2023
MysD codon optimized
Introduction
MAA analogues like shinorine or porphyra-334 is transformed from MG by a D-Ala-D-Ala ligaselike enzyme gene MysD[1].
Usage and Biology
We performed codon optimization on BBa_K4273013 (NlmysD) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysD catalyzes the fourth reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1003
References
- ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.