Difference between revisions of "Part:BBa K4765013"

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__NOTOC__
 
__NOTOC__
<partinfo>BBa_K4765007 short</partinfo>
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__TOC__
==Introduction==
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===Introduction===
 
MAA analogues like shinorine or porphyra-334 is transformed from MG by a D-Ala-D-Ala ligaselike enzyme gene MysD<ref>Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. ''The Journal of Organic Chemistry, 86''(16), 11160–11168.</ref>.
 
MAA analogues like shinorine or porphyra-334 is transformed from MG by a D-Ala-D-Ala ligaselike enzyme gene MysD<ref>Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. ''The Journal of Organic Chemistry, 86''(16), 11160–11168.</ref>.
  
==Usage and Biology==
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===Usage and Biology===
 
We performed codon optimization on [https://parts.igem.org/Part:BBa_K4273013 BBa_K4273013] (NlmysD) specifically for the ''Escherichia coli'' K12 strain, resulting in the creation of this part. The enzyme MysD catalyzes the fourth reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within ''E. coli''.
 
We performed codon optimization on [https://parts.igem.org/Part:BBa_K4273013 BBa_K4273013] (NlmysD) specifically for the ''Escherichia coli'' K12 strain, resulting in the creation of this part. The enzyme MysD catalyzes the fourth reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within ''E. coli''.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K4765007 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4765013 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
<partinfo>BBa_K4765007 parameters</partinfo>
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<partinfo>BBa_K4765013 parameters</partinfo>
 
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==References==
 
==References==
 
<references />
 
<references />

Revision as of 04:05, 30 September 2023


MysD codon optimized

Introduction

MAA analogues like shinorine or porphyra-334 is transformed from MG by a D-Ala-D-Ala ligaselike enzyme gene MysD[1].

Usage and Biology

We performed codon optimization on BBa_K4273013 (NlmysD) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysD catalyzes the fourth reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1003


References

  1. Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.