Difference between revisions of "Part:BBa K191006"

 
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<partinfo>BBa_K191006 short</partinfo>
 
<partinfo>BBa_K191006 short</partinfo>
  
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Coding sequence of a fused protein (LOV means light, oxygen, and voltage,represent a light sensitive protein domain whereas TAP means tryptophan-activated protein).
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Part BBa_K191006 contains coding sequence of the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain ( Lov means light, oxygen, voltage)and a regulatory domain (like Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore results in an increase or decrease of the regulatory domain's affinity for its DNA binding site.
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For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP Introduction LovTAP] on team EPF-Lausanne wiki
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 13:03, 21 October 2009

LOVTAP

Part BBa_K191006 contains coding sequence of the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain ( Lov means light, oxygen, voltage)and a regulatory domain (like Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore results in an increase or decrease of the regulatory domain's affinity for its DNA binding site.

For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP Introduction LovTAP] on team EPF-Lausanne wiki

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 535