Difference between revisions of "Part:BBa K4656008"
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After polymerization of butyrate and Lrp into binary complex, it can bind to PpchA promoter, thus activating the transcription and expression of pchA. The generated pchA can bind to the promoter PLEE1, thereby activating downstream CI repressor protein expression. This short genetic route can be used as a sensing module to sense the amount of butyrate in the gut. | After polymerization of butyrate and Lrp into binary complex, it can bind to PpchA promoter, thus activating the transcription and expression of pchA. The generated pchA can bind to the promoter PLEE1, thereby activating downstream CI repressor protein expression. This short genetic route can be used as a sensing module to sense the amount of butyrate in the gut. | ||
If the perceived content of butyrate in the gut is high, more CI repressor protein is produced, and if the perceived content of butyrate is low, less CI repressor protein is produced. By combining this gene route with the gene route of BBa_K4656007, when adding EGFP to the TPH1 and TDC of BBa_K4656007, the content of CI repressor protein can be determined by detecting fluorescence and then the content of butyrate in the intestine can be calculated. In addition, the protein expression of Plam-TPH1-TDC1 gene route can also be determined by special methods to detect 5-HT content in the intestine, and then the content of butyrate in the intestine can be estimated. | If the perceived content of butyrate in the gut is high, more CI repressor protein is produced, and if the perceived content of butyrate is low, less CI repressor protein is produced. By combining this gene route with the gene route of BBa_K4656007, when adding EGFP to the TPH1 and TDC of BBa_K4656007, the content of CI repressor protein can be determined by detecting fluorescence and then the content of butyrate in the intestine can be calculated. In addition, the protein expression of Plam-TPH1-TDC1 gene route can also be determined by special methods to detect 5-HT content in the intestine, and then the content of butyrate in the intestine can be estimated. | ||
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Revision as of 12:55, 10 August 2023
pchA-PpchA-PLEE1-CI
After polymerization of butyrate and Lrp into binary complex, it can bind to PpchA promoter, thus activating the transcription and expression of pchA. The generated pchA can bind to the promoter PLEE1, thereby activating downstream CI repressor protein expression. This short genetic route can be used as a sensing module to sense the amount of butyrate in the gut. If the perceived content of butyrate in the gut is high, more CI repressor protein is produced, and if the perceived content of butyrate is low, less CI repressor protein is produced. By combining this gene route with the gene route of BBa_K4656007, when adding EGFP to the TPH1 and TDC of BBa_K4656007, the content of CI repressor protein can be determined by detecting fluorescence and then the content of butyrate in the intestine can be calculated. In addition, the protein expression of Plam-TPH1-TDC1 gene route can also be determined by special methods to detect 5-HT content in the intestine, and then the content of butyrate in the intestine can be estimated.
