Difference between revisions of "Part:BBa K4656007:Experience"
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===Applications of BBa_K4656007=== | ===Applications of BBa_K4656007=== | ||
| + | To verify the feasibility of the metabolic module pathway, that is, the pathway for the production of sufficient tryptophan hydroxylase (TPH) and tryptophan decarboxylase (TDC) in engineered bacteria. | ||
| + | Using components BBa_K4656001, BBa_K4656002 and BBa_K4656006, we designed a gene route Plam-TPH1-TDC1-EGFP, cloned it into the target vector pET28a(+), and then transformed the engineered bacteria. The routes formed by these components can be used to verify the viability of metabolic modules. With untreated pET28a as the control group, we conducted experiments such as WB, fluorescence intensity (OD600) determination, gray value analysis, etc., to verify the feasibility of the metabolic module pathway. | ||
| + | <html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/wb.png"width="466" height="223"></center> | ||
| + | <p>WB result</p> | ||
| + | <html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/wb2.png"width="219" height="248"></center> | ||
| + | <p>WB result</p> | ||
| + | <html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/gray-value.png"width="392" height="292"></center> | ||
| + | <p>gray-value result</p> | ||
| + | <html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/metabolism-result.png"width="342.5" height="305"></center> | ||
| + | <p>metabolism result</p> | ||
| + | </html> | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K4656007 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K4656007 StartReviews</partinfo> | ||
Revision as of 15:05, 6 September 2023
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Applications of BBa_K4656007
To verify the feasibility of the metabolic module pathway, that is, the pathway for the production of sufficient tryptophan hydroxylase (TPH) and tryptophan decarboxylase (TDC) in engineered bacteria. Using components BBa_K4656001, BBa_K4656002 and BBa_K4656006, we designed a gene route Plam-TPH1-TDC1-EGFP, cloned it into the target vector pET28a(+), and then transformed the engineered bacteria. The routes formed by these components can be used to verify the viability of metabolic modules. With untreated pET28a as the control group, we conducted experiments such as WB, fluorescence intensity (OD600) determination, gray value analysis, etc., to verify the feasibility of the metabolic module pathway.
WB result
WB result
gray-value result
metabolism result
User Reviews
UNIQe73ebc6d736abb00-partinfo-00000001-QINU UNIQe73ebc6d736abb00-partinfo-00000002-QINU