Difference between revisions of "Part:BBa K4656004"

 
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<partinfo>BBa_K4656004 short</partinfo>
  
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PLEE1 is the promoter of the locus of enterocyte effacement which binds to pchA.
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Since the specific binding site of pchA and PLEE1 is still unknown, at present, researchers have purchased Escherichia coli genome from atcc and amplified PLEE1 with different fragments by PCR as candidates, and constructed 6 different kinds of plasmids. In the figure, +1 is the transcription start site TSS.
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"Additionally, the best performing plasmid, pC4S-P4, contains a small part of the ler gene in the PLEE1 promoter region."[1]  {The pC4S-P4:  pDMB,PpchA-pchA-PLEE1(-98+218bp)-gfp;CmR}
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4656004 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4656004 parameters</partinfo>
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Revision as of 02:18, 9 August 2023

PLEE1


PLEE1 is the promoter of the locus of enterocyte effacement which binds to pchA.

Since the specific binding site of pchA and PLEE1 is still unknown, at present, researchers have purchased Escherichia coli genome from atcc and amplified PLEE1 with different fragments by PCR as candidates, and constructed 6 different kinds of plasmids. In the figure, +1 is the transcription start site TSS.

"Additionally, the best performing plasmid, pC4S-P4, contains a small part of the ler gene in the PLEE1 promoter region."[1] {The pC4S-P4: pDMB,PpchA-pchA-PLEE1(-98+218bp)-gfp;CmR}

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]