Difference between revisions of "Part:BBa K4765903"

Revision as of 10:01, 7 August 2023

MysB codon optimized

Usage and Biology

We introduced a self-assembly synthetic adhesion system by transfecting this bio-brick into E. Coli. The bio-brick is composed of a surface display system(intimin) and the coding sequence of an antigen. The surface display system, which includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a beta-barrel for transmembrane insertion, possess the outer membrane anchoring of the antigen. The surface-displayed antigen can specifically interact with the nanobody produced by BBk_XXXXXX. In our project, we took full advantage of the Ag-Nb interaction to create a biofilm with a programmable physical structure. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 532

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K4765011 short</partinfo>
-We introduced a self-assembly synthetic adhesion system by transfecting this bio-brick into *E. Coli*. The bio-brick is composed of a surface display system(intimin) and the coding sequence of an antigen. The surface display system, which includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a beta-barrel for transmembrane insertion[^1], possess the outer membrane anchoring of the antigen[^2]. The surface-displayed antigen can specifically interact with the nanobody produced by  BBk_XXXXXX. In our project, we took full advantage of the Ag-Nb interaction to create a biofilm with a programmable physical structure[^3]
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+We introduced a self-assembly synthetic adhesion system by transfecting this bio-brick into E. Coli. The bio-brick is composed of a surface display system(intimin) and the coding sequence of an antigen. The surface display system, which includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a beta-barrel for transmembrane insertion, possess the outer membrane anchoring of the antigen. The surface-displayed antigen can specifically interact with the nanobody produced by  BBk_XXXXXX. In our project, we took full advantage of the Ag-Nb interaction to create a biofilm with a programmable physical structure.
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 <span class='h3bb'>Sequence and Features</span>