Difference between revisions of "Part:BBa K4765015"

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<partinfo>BBa_K4765000 short</partinfo>
 
<partinfo>BBa_K4765000 short</partinfo>
  
AnAFP is an antifreeze protein derived from *Ammopiptanthus nanus*. This low-temperature-induced protein is predicted to protect the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions. We performed codon optimization of the AnAFP sequence for E.coli K12 to enable its expression in Escherichia coli, resulting in the production of antifreeze effects.
 
  
 
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===Usage and Biology===
 
===Usage and Biology===
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AnAFP is an antifreeze protein derived from *Ammopiptanthus nanus*. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions<ref>Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang,  S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of  antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold  tolerance in Escherichia coli and tobacco. *Gene*, *539*(1), 132–140.https://doi.org/10.1016/j.gene.2014.01.013</ref>. We heterologously expressed codon-optimized AnAFP in *Escherichia coli*, endowing it with antifreeze capability.
  
 
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<partinfo>BBa_K4765000 parameters</partinfo>
 
<partinfo>BBa_K4765000 parameters</partinfo>
 
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==References==
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<references />

Revision as of 07:58, 5 August 2023


image demonstration


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References