Difference between revisions of "Part:BBa K4727005:Design"

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===Design Notes===
 
===Design Notes===
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Codon optimised with Codonator3000 (iGEM Sydney 2019) for expression in E. coli and for in vitro synthesis, made RFC[10] compatible, added a start codon and stop codon
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Codon optimised with Codonator3000 (iGEM Sydney 2019) [1] for expression in E. coli and for in vitro synthesis, made RFC[10] compatible, added a start codon and stop codon
  
  

Latest revision as of 13:37, 25 July 2023


Pf3 Pseudomonas Phage Gene 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 576
    Illegal BsaI site found at 900


Design Notes

Codon optimised with Codonator3000 (iGEM Sydney 2019) [1] for expression in E. coli and for in vitro synthesis, made RFC[10] compatible, added a start codon and stop codon


Source

Pf3 bacteriophage deposited genome NCBI ID: NC_074763

References

[1] CODONATOR 3000 Copyright © 2019, Tristan Ofner, Fahad Ali, Merrie Caruana, Benj Gonzaga, Nathan Hawkins, Isobel McGrath, Emma Todd.