Difference between revisions of "Part:BBa K4883000"
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− | + | ADE4 encodes for phosphoribosylpyrophosphate amidotransferase in S. cerevisiae. This enzyme catalyzes the first step of the purine nucleotide biosynthetic pathway, directly forming inosine monophosphate (IMP) (Nieto & Woods, 1983). | |
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<partinfo>BBa_K4883000 parameters</partinfo> | <partinfo>BBa_K4883000 parameters</partinfo> | ||
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+ | <!-- Add more about the biology of this part here --> | ||
+ | ===Usage and Biology=== | ||
+ | ADE4 helps to synthesize IMP. IMP is the precursor of GTP, and GTP is the precusor of vitamin B2 (Kowalski et al., 2008; Gudipati et al., 2014). Overexpression of ADE4 could lead to overproduction of vitamin B2 in S. cerevisiae. | ||
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+ | <html> | ||
+ | <body> | ||
+ | <figure> | ||
+ | <div class = "center"> | ||
+ | <center><img src = "https://static.igem.wiki/teams/4883/wiki/part-bba-k4883000-1.png" style = "width:480px"></center> | ||
+ | </div> | ||
+ | <figcaption><center>Figure 1. Gel electrophoresis of colony PCR products for verification of correct transformation of plasmid pCEV-G4-ADE4-Km into E. coli DH5α </center></figcaption> | ||
+ | </figure> | ||
+ | </body> | ||
+ | </html> | ||
+ | |||
+ | ==References== | ||
+ | Gudipati, V.; Koch, K.; Lienhart, W.-D.; Macheroux, P. The Flavoproteome of the Yeast Saccharomyces Cerevisiae. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 2014, 1844 (3), 535–544. DOI:10.1016/j.bbapap.2013.12.015. | ||
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+ | Kowalski, D.; Pendyala, L.; Daignan-Fornier, B.; Howell, S. B.; Huang, R.-Y. Dysregulation of Purine Nucleotide Biosynthesis Pathways Modulates Cisplatin Cytotoxicity in Saccharomyces Cerevisiae. Molecular Pharmacology 2008, 74 (4), 1092–1100. DOI:10.1124/mol.108.048256. | ||
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+ | Nieto DJ, Woods RA. Studies on mutants affecting amidophosphoribosyltransferase activity in Saccharomyces cerevisiae. Can J Microbiol. 1983 Jun;29(6):681-8. doi: 10.1139/m83-111. PMID: 6349762. |
Latest revision as of 01:38, 6 October 2023
ADE4, phosphoribosylpyrophosphate amidotransferase from Saccharomyces cerevisiae
ADE4 encodes for phosphoribosylpyrophosphate amidotransferase in S. cerevisiae. This enzyme catalyzes the first step of the purine nucleotide biosynthetic pathway, directly forming inosine monophosphate (IMP) (Nieto & Woods, 1983).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
ADE4 helps to synthesize IMP. IMP is the precursor of GTP, and GTP is the precusor of vitamin B2 (Kowalski et al., 2008; Gudipati et al., 2014). Overexpression of ADE4 could lead to overproduction of vitamin B2 in S. cerevisiae.
References
Gudipati, V.; Koch, K.; Lienhart, W.-D.; Macheroux, P. The Flavoproteome of the Yeast Saccharomyces Cerevisiae. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 2014, 1844 (3), 535–544. DOI:10.1016/j.bbapap.2013.12.015.
Kowalski, D.; Pendyala, L.; Daignan-Fornier, B.; Howell, S. B.; Huang, R.-Y. Dysregulation of Purine Nucleotide Biosynthesis Pathways Modulates Cisplatin Cytotoxicity in Saccharomyces Cerevisiae. Molecular Pharmacology 2008, 74 (4), 1092–1100. DOI:10.1124/mol.108.048256.
Nieto DJ, Woods RA. Studies on mutants affecting amidophosphoribosyltransferase activity in Saccharomyces cerevisiae. Can J Microbiol. 1983 Jun;29(6):681-8. doi: 10.1139/m83-111. PMID: 6349762.