Difference between revisions of "Part:BBa J435320"

 
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_lacZ pDest cloning insert for FreeGenes plasmid for building Type IIS TU, drops out with BsaI
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FLANKED 5' fusion site: GCTT, 3' fusion site: GGAG
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Source organism: N/A, Target organism: Escherichia coli
 
Source organism: N/A, Target organism: Escherichia coli

Latest revision as of 13:41, 13 June 2023


AE_lacZ pDest


_lacZ pDest cloning insert for FreeGenes plasmid for building Type IIS TU, drops out with BsaI

Source organism: N/A, Target organism: Escherichia coli


Citation: https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit#gid=1530011293

ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_003375.html

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 250
    Illegal XbaI site found at 223
    Illegal PstI site found at 211
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 250
    Illegal PstI site found at 211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 250
    Illegal BamHI site found at 229
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 250
    Illegal XbaI site found at 223
    Illegal PstI site found at 211
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 250
    Illegal XbaI site found at 223
    Illegal PstI site found at 211
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 607
    Illegal BsaI.rc site found at 2