Difference between revisions of "Part:BBa K4119015"
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<p style="font-size: 180%; font-weight: bold;">Results:</p> | <p style="font-size: 180%; font-weight: bold;">Results:</p> | ||
| − | <p>The | + | <p>The new promoter thlAF3 has a satisfatory performence. It is inhibited in aerobic condition while strengthed in microaerobic condition.</p> |
| − | <p><img src="https://static.igem.wiki/teams/4119/wiki/jt-files/h2/ | + | <p><img src="https://static.igem.wiki/teams/4119/wiki/jt-files/h2/img11.png" width="80%" height="80%"></p> |
<div align="center"> | <div align="center"> | ||
| − | <strong>Fig.2. Expression effect of PthlA and | + | <strong>Fig.2. Expression effect of PthlA and PthlAF3-bs2 at different oxygen concentrations</strong> |
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4119015 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4119015 SequenceAndFeatures</partinfo> | ||
Latest revision as of 04:00, 14 October 2022
PthlA-FNRbindingsite-3
PthlAF3 was modified based on the PthlA constitutive promoter from Clostridium acetobutyricum.A FNR binding site was inserted at the distance of 3 bp from the - 35 region of the PthlA promoter.
Results:
The new promoter thlAF3 has a satisfatory performence. It is inhibited in aerobic condition while strengthed in microaerobic condition.
Fig.2. Expression effect of PthlA and PthlAF3-bs2 at different oxygen concentrations
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]