Difference between revisions of "Part:BBa K4260030"
 
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<br><strong>Type:</strong>Basic part</br>
 
<br><strong>Type:</strong>Basic part</br>
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<br><strong>Designed by:</strong>Claudia Angélica García Alonso</br>
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<br><strong>Group:</strong>iGEM_TecCEM</br>
 
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<p align = "justify">This part codes a transcriptional activator, specific for the transcription of the coding gene of Isoeugenol monooxygenase, an enzyme that helps the direct conversion of isoeugenol to vanillin. It has been proven that the expression of this gene, signinficantly increases the expression of Isouegenol monooxygenase (Ryu, J. Y., Seo, J., Ahn, J. H., Sadowsky, M. J., & Hur, H. G. (2012). Transcriptional control of the isoeugenol monooxygenase of Pseudomonas nitroreducens Jin1 in Escherichia coli. Bioscience, biotechnology, and biochemistry, 76(10), 1891-1896) .
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<p align = "justify">This part codes a transcriptional activator, specific for the transcription of the coding gene of Isoeugenol monooxygenase, an enzyme that helps the direct conversion of isoeugenol to vanillin. It has been proven that the expression of this gene, significantly increases the expression of Isoeugenol monooxygenase (Ryu, J. Y., Seo, J., Ahn, J. H., Sadowsky, M. J., & Hur, H. G. (2012). Transcriptional control of the isoeugenol monooxygenase of Pseudomonas nitroreducens Jin1 in Escherichia coli. Bioscience, biotechnology, and biochemistry, 76(10), 1891-1896) .
  
  
 
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<img style="vertical-align: bottom;)" width=90% src="https://parts.igem.org/File:IemR.jpeg#file">
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<img style="vertical-align: bottom;)" width=90% src="https://static.igem.org/mediawiki/parts/b/bf/IemR.jpeg">
 
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<img style="vertical-align: bottom;)" width=50% src="https://static.igem.org/mediawiki/parts/b/b6/IemR3D.jpeg">
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<center><strong>Figure. 2 </strong> IemR docking.</center>
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<H3>Usage and Biology</H3>
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[[File:IemR_cosa_roja.jpeg|300px|thumb|right|<i><b>Figure.3:</b>Surface charge 3D model.</i>]]
 
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<H3>Design</H3>
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<p align = "justify">In the picture (fig 3), it is seen that in the middle of the structure, the value of the potential field is mostly negative (red), whilst the exterior of the molecule appears to be blue which means that the protein would present a higher affinity with some other target molecule. Nevertheless, the charge that domains in this molecule are neutral (white).
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[[File:Polaridad_iemR_cosa_morada.jpeg|300px|thumb|left|<i><b>Figure.4:</b>IemR charges.</i>]]
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<p align = "justify">In the picture, it is seen that mostly in the center of the molecular protein has more non-polar activity, whether the value has bigger polar residues the activity increases its neutral activity then its positive polar activity, so the extremes of the protein have more positive polar activity. 
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<H4><em>Application</em></H4>
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<p align = "justify">For more information please check <a href="https://parts.igem.org/Part:BBa_K4260006">https://parts.igem.org/Part:BBa_K4260006</a> . </p>
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<H4><em>Biosafety</em></H4>
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<p align = "justify">Although this coding sequence comes from a Pseudomonas bacteria,  it is not associated with the pathogenicity of the microorganism itself. </p>
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<hr>
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<H3>References</H3>
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<p align = "justify">[1] Ryu, J. Y., Seo, J., Ahn, J. H., Sadowsky, M. J., & Hur, H. G. (2012). Transcriptional control of the isoeugenol monooxygenase of Pseudomonas nitroreducens Jin1 in Escherichia coli. Bioscience, biotechnology, and biochemistry, 76(10), 1891-1896.</p>

Latest revision as of 03:16, 14 October 2022


IemR: Transcriptional activator of Iso


Type:Basic part

Designed by:Claudia Angélica García Alonso

Group:iGEM_TecCEM


This part codes a transcriptional activator, specific for the transcription of the coding gene of Isoeugenol monooxygenase, an enzyme that helps the direct conversion of isoeugenol to vanillin. It has been proven that the expression of this gene, significantly increases the expression of Isoeugenol monooxygenase (Ryu, J. Y., Seo, J., Ahn, J. H., Sadowsky, M. J., & Hur, H. G. (2012). Transcriptional control of the isoeugenol monooxygenase of Pseudomonas nitroreducens Jin1 in Escherichia coli. Bioscience, biotechnology, and biochemistry, 76(10), 1891-1896) .


Figure. 1 Design scheme of BBa_K4260030.


Figure. 2 IemR docking.

Usage and Biology

Figure.3:Surface charge 3D model.

In the picture (fig 3), it is seen that in the middle of the structure, the value of the potential field is mostly negative (red), whilst the exterior of the molecule appears to be blue which means that the protein would present a higher affinity with some other target molecule. Nevertheless, the charge that domains in this molecule are neutral (white).

Figure.4:IemR charges.


In the picture, it is seen that mostly in the center of the molecular protein has more non-polar activity, whether the value has bigger polar residues the activity increases its neutral activity then its positive polar activity, so the extremes of the protein have more positive polar activity.



Application

For more information please check https://parts.igem.org/Part:BBa_K4260006 .

Biosafety

Although this coding sequence comes from a Pseudomonas bacteria, it is not associated with the pathogenicity of the microorganism itself.

References

[1] Ryu, J. Y., Seo, J., Ahn, J. H., Sadowsky, M. J., & Hur, H. G. (2012). Transcriptional control of the isoeugenol monooxygenase of Pseudomonas nitroreducens Jin1 in Escherichia coli. Bioscience, biotechnology, and biochemistry, 76(10), 1891-1896.