Difference between revisions of "Part:BBa K203114"

Revision as of 16:11, 15 October 2009 (view source) Registry (Talk | contribs)		Revision as of 10:08, 17 October 2009 (view source) Larsplus (Talk | contribs) Newer edit →
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	<partinfo>BBa_K203114 short</partinfo>		<partinfo>BBa_K203114 short</partinfo>
−	To be updated	+	A synthetic promoter upregulated by pPAR&gamma; activation. Manufactured by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR].
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	===Usage and Biology===		===Usage and Biology===
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		+	[[Image:HD09_ppary.png|thumb|left|300px|'''pPAR&gamma; activation'''. During fatty diet, fatty acids are converted to prostaglandins by oxygenases, which activate pPAR&gamma;. Image published under [http://en.wikipedia.org/wiki/File:PPAR-diagram.png GNU Free documentation license.]]] Peroxisome proliferator-activated receptor γ (PPAR γ) is a transcription factor belonging to the family of nuclear receptors. PPAR γ plays an important role in glucose metabolism and fatty acid storage. PPAR γ is basically activated by ligands like the prostaglandin PGJ2 and through dimerization with retinoid X receptor (RXR). The activated heterodimer binds to the DNA consensus sequence AGGTCANAGGTCA resulting in an increased or decreased transcription of the appropriate gene. The genes activated by PPAR γ initiate the uptake of fatty acids and differentiation of cells to adipocytes. Besides its function in metabolism, PPAR γ was also shown to be correlated with several diseases such as cancer and diabetes. Activation of PPAR γ by synthetic PPAR γ ligands result in an increased glucose uptake. These syntethtic ligands are therefore promising agents in diabetes II treatment.  Another synthetic ligand of PPAR γ is able to inhibit the proliferation of different cancer types. For references, see [http://2009.igem.org/Team:Heidelberg/Eucaryopedia Heidelberg 2009's Eukaryopedia].
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	===Functional Parameters===		===Functional Parameters===
	<partinfo>BBa_K203114 parameters</partinfo>		<partinfo>BBa_K203114 parameters</partinfo>
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		+	pPAR&gamma; was induced by 5µM Thiazolidinedione in [http://2009.igem.org/Team:Heidelberg/Eucaryopedia#U2-OS U2OS cells]. Promoter activtiy was then roughly characterized in [http://2009.igem.org/Team:Heidelberg/Measurement REU] by TECAN (automated plate fluorescence reader). This promoter corresponds to clone &gamma;L9.
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		+	[[Image:HD09_ppary|thumb|none|400px|'''Characterization of two pPAR&gamma; responsive promoters.''' Clones created by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR] were screened and two promising clones were characterized by triple TECAN reads.]]
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Revision as of 10:08, 17 October 2009

PPARγ-regulated promoter 1

A synthetic promoter upregulated by pPARγ activation. Manufactured by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR].

Usage and Biology

Peroxisome proliferator-activated receptor γ (PPAR γ) is a transcription factor belonging to the family of nuclear receptors. PPAR γ plays an important role in glucose metabolism and fatty acid storage. PPAR γ is basically activated by ligands like the prostaglandin PGJ2 and through dimerization with retinoid X receptor (RXR). The activated heterodimer binds to the DNA consensus sequence AGGTCANAGGTCA resulting in an increased or decreased transcription of the appropriate gene. The genes activated by PPAR γ initiate the uptake of fatty acids and differentiation of cells to adipocytes. Besides its function in metabolism, PPAR γ was also shown to be correlated with several diseases such as cancer and diabetes. Activation of PPAR γ by synthetic PPAR γ ligands result in an increased glucose uptake. These syntethtic ligands are therefore promising agents in diabetes II treatment. Another synthetic ligand of PPAR γ is able to inhibit the proliferation of different cancer types. For references, see [http://2009.igem.org/Team:Heidelberg/Eucaryopedia Heidelberg 2009's Eukaryopedia].

Sequence and Features

Functional Parameters

pPARγ was induced by 5µM Thiazolidinedione in [http://2009.igem.org/Team:Heidelberg/Eucaryopedia#U2-OS U2OS cells]. Promoter activtiy was then roughly characterized in [http://2009.igem.org/Team:Heidelberg/Measurement REU] by TECAN (automated plate fluorescence reader). This promoter corresponds to clone γL9.