Difference between revisions of "Part:BBa K4438161:Experience"
 
Line 4: Line 4:
 
===Applications of BBa_K4438161===
 
===Applications of BBa_K4438161===
 
[[File:T--IISER-Tirupati_India--Fluroscence_miR-222_(30_min).png]]
 
[[File:T--IISER-Tirupati_India--Fluroscence_miR-222_(30_min).png]]
−
The fluorescence readout shown above observes a spike in the highest concentration of dye and miDNA. This supports the fact that the formation of G-quadruplex is facilitated by the addition of the biomarker.
+
 
 
[[File:T--IISER-Tirupati_India--Fluroscence_miR_222_(1_hr).png]]
 
[[File:T--IISER-Tirupati_India--Fluroscence_miR_222_(1_hr).png]]
−
The fluorescence readout shown above observes a spike in the highest concentration of dye and miDNA. This supports the fact that the formation of G-quadruplex is facilitated by the addition of the biomarker.
+
Depicting fluorescence readout of FASTmiR-222-D2 after 30 mins and 1 hour incubation respectively.Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA
 +
 
 +
Explanation: After 30mins of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-222 sensor was increased in concentration. An average fold-change of 2.69 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 6.048 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.
 +
 
 +
 
 +
After 1 hour, an average fold-change of 3.07 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 7.423 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.
 +
 
 
===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K4438161 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K4438161 StartReviews</partinfo>

Latest revision as of 02:58, 14 October 2022


Applications of BBa_K4438161

T--IISER-Tirupati India--Fluroscence miR-222 (30 min).png

T--IISER-Tirupati India--Fluroscence miR 222 (1 hr).png Depicting fluorescence readout of FASTmiR-222-D2 after 30 mins and 1 hour incubation respectively.Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA

Explanation: After 30mins of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-222 sensor was increased in concentration. An average fold-change of 2.69 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 6.048 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.


After 1 hour, an average fold-change of 3.07 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 7.423 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.

User Reviews

UNIQ92922d0d9734b850-partinfo-00000000-QINU UNIQ92922d0d9734b850-partinfo-00000001-QINU