Difference between revisions of "Part:BBa K4390058"
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The green fluorescent protein (GFP) is a protein that can produce bright green colour. Due to these properties, it has always been used as reporter of protein expression. Protein fused with sfGFP will turn the colony green if expressed, which can be used to identify the ability of certain genes to be expressed in other organism like E. coli. | The green fluorescent protein (GFP) is a protein that can produce bright green colour. Due to these properties, it has always been used as reporter of protein expression. Protein fused with sfGFP will turn the colony green if expressed, which can be used to identify the ability of certain genes to be expressed in other organism like E. coli. | ||
| − | This part contains sfGFP fused with 6x-his tag. This part is used as control for his-tagged MT transformation and expressions. | + | This part contains sfGFP fused with 6x-his tag. This part is used to check whether the assemply is successful, and also act as control for his-tagged MT transformation and expressions. |
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==<span class='h3bb'>Sequence and Features</span>== | ==<span class='h3bb'>Sequence and Features</span>== | ||
<partinfo>BBa_K4390058 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4390058 SequenceAndFeatures</partinfo> | ||
Revision as of 15:57, 13 October 2022
His-tagged sfGFP expression construct (JUMP assembled)
This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.
Usage and Biology
The green fluorescent protein (GFP) is a protein that can produce bright green colour. Due to these properties, it has always been used as reporter of protein expression. Protein fused with sfGFP will turn the colony green if expressed, which can be used to identify the ability of certain genes to be expressed in other organism like E. coli.
This part contains sfGFP fused with 6x-his tag. This part is used to check whether the assemply is successful, and also act as control for his-tagged MT transformation and expressions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 128