Difference between revisions of "Part:BBa K200028"

 
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<partinfo>BBa_K200028 short</partinfo>
 
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Site directed mutagenesis was carried out to change a glutamine residue to a serine to make the PAH enzyme protease resistant. This will allow safe passage through the stomach and gut in order to carry out function as required by Imperial College's 2009 iGEM project, The E.ncapsulator.
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Site directed mutagenesis was carried out to change a serine residue (Ser16) to a glutamine to make the PAH enzyme protease resistant. This will allow safe passage through the stomach and gut in order to carry out function as required by Imperial College's 2009 iGEM project, [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>].
  
 
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Revision as of 13:00, 18 October 2009

Protease resistant PAH

Site directed mutagenesis was carried out to change a serine residue (Ser16) to a glutamine to make the PAH enzyme protease resistant. This will allow safe passage through the stomach and gut in order to carry out function as required by Imperial College's 2009 iGEM project, [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]