Difference between revisions of "Part:BBa K4438168:Experience"
 
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===Applications of BBa_K4438168===
 
===Applications of BBa_K4438168===
 
[[File:T--IISER-Tirupati_India--fluroscence_miR-146_(30_min).png]]
 
[[File:T--IISER-Tirupati_India--fluroscence_miR-146_(30_min).png]]
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The fluorescence readout shown above observes a spike in the highest concentration of dye and miDNA. This supports the fact that the formation of G-quadruplex is facilitated by the addition of the biomarker.
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[[File:T--IISER-Tirupati_India--fluroscence_miR-146-(1_hr).png]]
 
[[File:T--IISER-Tirupati_India--fluroscence_miR-146-(1_hr).png]]
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The fluorescence readout shown above observes a spike in the highest concentration of dye and miDNA. This supports the fact that the formation of G-quadruplex is facilitated by the addition of the biomarker.
+
 
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Depicting the fluorescence readout of FASTmiR-146a-D4 (design 4) after 30 mins and 1 hour incubation respectively. Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA
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Explanation: After 30 minutes of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-146a sensor was increased in concentration. An average fold-change of 1.327 was observed after the addition of miDNA-146a throughout a range of DNA concentrations, with a maximum fold-change of 3.319 observed at a DNA concentration of 1.8 μM and a FASTmiR-146a concentration of 90 nM.
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While after 1 hour of incubation with 5X sensor buffer and dye, an average fold-change of 1.41 was observed after the addition of miDNA-146a throughout a range of DNA concentrations, with a maximum fold-change of 3.311 observed at a DNA concentration of 1.8 μM and a FASTmiR-146a concentration of 90 nM.
 
===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K4438168 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K4438168 StartReviews</partinfo>

Latest revision as of 03:06, 14 October 2022


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Applications of BBa_K4438168

T--IISER-Tirupati India--fluroscence miR-146 (30 min).png

T--IISER-Tirupati India--fluroscence miR-146-(1 hr).png

Depicting the fluorescence readout of FASTmiR-146a-D4 (design 4) after 30 mins and 1 hour incubation respectively. Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA

Explanation: After 30 minutes of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-146a sensor was increased in concentration. An average fold-change of 1.327 was observed after the addition of miDNA-146a throughout a range of DNA concentrations, with a maximum fold-change of 3.319 observed at a DNA concentration of 1.8 μM and a FASTmiR-146a concentration of 90 nM.


While after 1 hour of incubation with 5X sensor buffer and dye, an average fold-change of 1.41 was observed after the addition of miDNA-146a throughout a range of DNA concentrations, with a maximum fold-change of 3.311 observed at a DNA concentration of 1.8 μM and a FASTmiR-146a concentration of 90 nM.

User Reviews

UNIQ7298758a855406ff-partinfo-00000000-QINU UNIQ7298758a855406ff-partinfo-00000001-QINU