Difference between revisions of "Part:BBa K4438101:Experience"
 
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===Applications of BBa_K4438101===
 
===Applications of BBa_K4438101===
 
[[File:T--IISER-Tirupati_India--Fluroscence_miR-222_(30_min).png]]
 
[[File:T--IISER-Tirupati_India--Fluroscence_miR-222_(30_min).png]]
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The above graphs show a significant fold change when the miDNA is added to the reaction mixture. This suggests the formation of a stable G-quadruplex for DFHBI binding is facilitated by the miDNA. This reading is taken after 30 mins of incubation.
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[[File:T--IISER-Tirupati_India--Fluroscence_miR_222_(1_hr).png]]
 
[[File:T--IISER-Tirupati_India--Fluroscence_miR_222_(1_hr).png]]
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The above graphs show a significant fold change when the miDNA is added to the reaction mixture. This suggests the formation of a stable G-quadruplex for DFHBI binding is facilitated by the miDNA. This reading is taken after 1 hour of incubation.
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Depicting fluorescence readout of FASTmiR-222-D2 after 30 mins and 1 hour incubation respectively.Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA
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Explanation: After 30mins of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-222 sensor was increased in concentration. An average fold-change of 2.69 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 6.048 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.
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After 1 hour, an average fold-change of 3.07 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 7.423 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.
  
  

Latest revision as of 02:58, 14 October 2022


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Applications of BBa_K4438101

T--IISER-Tirupati India--Fluroscence miR-222 (30 min).png

T--IISER-Tirupati India--Fluroscence miR 222 (1 hr).png Depicting fluorescence readout of FASTmiR-222-D2 after 30 mins and 1 hour incubation respectively.Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA

Explanation: After 30mins of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-222 sensor was increased in concentration. An average fold-change of 2.69 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 6.048 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.


After 1 hour, an average fold-change of 3.07 was observed after the addition of miDNA-222 throughout a range of DNA concentrations, with a maximum fold-change of 7.423 observed at a DNA concentration of 1 μM and a FASTmiR-222 concentration of 70 nM.


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