Difference between revisions of "Part:BBa K4390023"

Revision as of 15:43, 12 October 2022

Cellulose Binding Domain :: Gly-Ser Linker

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

This is an O part containing a cellulose-binding domain and a glycine-serine linker. The cellulose-binding domain allows for the expressed level 1 construct to bind to cellulose, while the glycine-serine linker introduces a short unstructured domain, minimising the risk of the cellulose-binding domain interfering with the activity of other parts. The 2022 Edinburgh-UHAS iGEM team used this part to add metallothioneins to a cellulose hydrogel.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4390023 short</partinfo>
+'''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]'''
 This is an O part containing a cellulose-binding domain and a glycine-serine linker. The cellulose-binding domain allows for the expressed level 1 construct to bind to cellulose, while the glycine-serine linker introduces a short unstructured domain, minimising the risk of the cellulose-binding domain interfering with the activity of other parts. The 2022 Edinburgh-UHAS iGEM team used this part to add metallothioneins to a cellulose hydrogel.