Difference between revisions of "Part:BBa K243017:Design"
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===Design Notes=== | ===Design Notes=== | ||
The choice of the linker, lipocalin tag and purification tag allows us several different combinations. | The choice of the linker, lipocalin tag and purification tag allows us several different combinations. | ||
| − | + | [https://static.igem.org/mediawiki/parts/0/04/Strepdigasplitfoka.txt Commented GenBank file] | |
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===Source=== | ===Source=== | ||
Revision as of 18:06, 20 October 2009
Strep-DigA-Split Linker-Fok_a
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 278
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1132
Design Notes
The choice of the linker, lipocalin tag and purification tag allows us several different combinations. Commented GenBank file
Source
Combined the parts by serial cloning steps.