Difference between revisions of "Part:BBa K4438200"

 
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Spinach 2.2 is a mutant version of Spinach 2.1(BBa_K1330000), made to increase the fluorescent intensity given out by the sptamer on binding with DFHBI.
 
Spinach 2.2 is a mutant version of Spinach 2.1(BBa_K1330000), made to increase the fluorescent intensity given out by the sptamer on binding with DFHBI.
  
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===Usage and Biology===
 
===Usage and Biology===
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The free energy of the thermodynamic ensemble for the sequence below is predicted to be -34.44 kcal/mol.The tetraloop is highlighted in red text and the inversions are underlined. The sequences highlighted in bold are the aptamer sequence and those flanking are the tRNA scaffold sequence, tRNALys3. The mutation and inversions are indicated in the RNAfold structures using blue circles.
  
 
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Revision as of 14:34, 12 October 2022


Spinach2.2

Spinach 2.2 is a mutant version of Spinach 2.1(BBa_K1330000), made to increase the fluorescent intensity given out by the sptamer on binding with DFHBI.


Usage and Biology

The free energy of the thermodynamic ensemble for the sequence below is predicted to be -34.44 kcal/mol.The tetraloop is highlighted in red text and the inversions are underlined. The sequences highlighted in bold are the aptamer sequence and those flanking are the tRNA scaffold sequence, tRNALys3. The mutation and inversions are indicated in the RNAfold structures using blue circles.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]