Difference between revisions of "Part:BBa K4361116"

Revision as of 14:35, 12 October 2022

GHB / GBL biosensor reporter, deletion in Blc operator

This part shows the GHB / GBL reporter system designed by the Bielefeld-CeBiTec iGEM 2015 team (Part:BBa_K1758376) but with a deletion in the blc operator sequence. Deletion is inserted with primers Part:BBa_K4361112 and Part:BBa_K4361113.

It is known from literature that this mutation disrupts the binding between BlcR and the blc operator sequence [1]. This way BlcR cannot inhibit downstream gene expression.

The mutated blc operator sequence is incorperated in a superfolded GFP production plasmid, seePart:BBa_K4361115.