Difference between revisions of "Part:BBa K243010:Design"

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(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The choice of the linker, lipocalin tag and purification tag allows us serval different combinations.
 
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The combiantion of HisTag linked with a FluA Tag connect by the Split Linker approved as a good way to express the inactive protein domain[https://parts.igem.org/wiki/index.php?title=Part:BBa_K243001] of our universal endonuclease. 
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The parts are fused with RFC25.
  
 
===Source===
 
===Source===

Revision as of 17:50, 20 October 2009

His-FluA-Split Linker-Fok_i


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The combiantion of HisTag linked with a FluA Tag connect by the Split Linker approved as a good way to express the inactive protein domain[1] of our universal endonuclease. The parts are fused with RFC25.

Source

Combined the parts by serial cloning steps.

References