Difference between revisions of "Part:BBa K4439009"

Line 2: Line 2:
  
 
==Abstract==
 
==Abstract==
 +
The GST-N[AS]8C-CBD-10xHis protein was implemented in a pET28a vector. This protein could allow to test the polymerisation of [AS] modules present in recombinant green lacewing silk proteins to create a very specific binding between two proteins.
 +
 +
The assembly compatibility of this part surprised the team: we think that we don't have any assembly compatibility because of the fact that we did a small mutation on the CBD sequence that is not taken into account in the part we used ([[Part:BBa K4439015]]).
 +
 +
The mutation is at bp153 where a C was changed into a T, which is a silent mutation.
  
 
==Sequence and Features==
 
==Sequence and Features==
 
<partinfo>BBa_K4439009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4439009 SequenceAndFeatures</partinfo>

Revision as of 14:37, 12 October 2022

GST-N[AS]8C-CBD-10xHis

Abstract

The GST-N[AS]8C-CBD-10xHis protein was implemented in a pET28a vector. This protein could allow to test the polymerisation of [AS] modules present in recombinant green lacewing silk proteins to create a very specific binding between two proteins.

The assembly compatibility of this part surprised the team: we think that we don't have any assembly compatibility because of the fact that we did a small mutation on the CBD sequence that is not taken into account in the part we used (Part:BBa K4439015).

The mutation is at bp153 where a C was changed into a T, which is a silent mutation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2592
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2592
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2592
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2592
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 82