Difference between revisions of "Part:BBa K4119003"
 
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<partinfo>BBa_K4119003 short</partinfo>
 
<partinfo>BBa_K4119003 short</partinfo>
  
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In this part of our project, the key promoter vgb was a microaerobic induced promoter of Vitreoscilla hemoglobin gene. Considering the gene compatibility difference between different host bacteria, we designed the pMTL-Pvgb-bs2 plasmid to determine whether the promoter vgb could work normally in Clostridium tyrobutyricum by detecting the fluorescent expression intensity of fluorescent protein Bs2.
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it's Pvgb-Bs2-Cpa fdx terminator
  
 
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     <p>The test group: the fluorescence intensity is relatively high</p>
 
     <p>The test group: the fluorescence intensity is relatively high</p>
 
     <p>The control group: the fluorescence intensity is lower compared to the test group</p>
 
     <p>The control group: the fluorescence intensity is lower compared to the test group</p>
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     <p>The engineered bacteria bearing pMTL-Pvgb-bs2, pMTL-Pthl-bs2 and pMTL82151 plasmids were precultured, inoculated into LB culture medium with different dissolved oxygen, and cultured to OD600 ~0.7 and ~1.2, respectively. The subsequent operation was as recorded by protocol, measuring the fluorescence intensity, collating and the data was analyzed. </p>
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     <p><img src="https://static.igem.wiki/teams/4119/wiki/jt-files/h2/img7.png" width="80%" height="80%"></p>
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    <p>The vgb promoter performed a low expression under aerobic conditions, while under microaerobic conditions, it expressed 10.2 folds increased on fluorescence intensity comparing with the constituent promoter, which is exactly as expected.</p>
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     <p><img src="https://static.igem.wiki/teams/4119/wiki/jt-files/h2/img8.png" width="80%" height="80%"></p>
 
     <p><img src="https://static.igem.wiki/teams/4119/wiki/jt-files/h2/img8.png" width="80%" height="80%"></p>
 
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Latest revision as of 14:35, 12 October 2022


Pvgb-Bs2-Cpa fdx terminator

it's Pvgb-Bs2-Cpa fdx terminator


Document

Results:

The test group: the fluorescence intensity is relatively high

The control group: the fluorescence intensity is lower compared to the test group

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 350
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 350
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 350
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 350
  • 1000
    COMPATIBLE WITH RFC[1000]