Difference between revisions of "Part:BBa K4511007"

Revision as of 14:29, 12 October 2022

Reporter device with J23106 , B0032, and GFP for dtRNA1 strength measurement

This part is a measurement device for degradation-tuning RNAs, which contains medium strength J23106 promoter, medium RBS B0032, and a reporter protein coding sequence GFP. The effectiveness of dtRNA is evaluated by measuring GFP fluorescence and bacterial growth curve. It could be used as an inter-lab study device to compare experimental conditions among different labs. This plasmid could also be directly transformed into other species or strains of E.coli to study the effectiveness of dtRNA on another chassis.

Improvement by 2022 team HUS_United

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 753

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 This part is a measurement device for degradation-tuning RNAs, which contains medium strength J23106 promoter, medium RBS B0032, and a reporter protein coding sequence GFP. The effectiveness of dtRNA is evaluated by measuring GFP fluorescence and bacterial growth curve. It could be used as an inter-lab study device to compare experimental conditions among different labs. This plasmid could also be directly transformed into other species or strains of E.coli to study the effectiveness of dtRNA on another chassis.
+==Improvement by 2022 team HUS_United==
 <!-- Add more about the biology of this part here