Difference between revisions of "Part:BBa K4169016"

Revision as of 13:25, 12 October 2022

produce DMADH

After expressing, it'll produce dimethylamine dehydrogenase (DMADH). The enzyme DMADH is an iron–sulfur flavoprotein which catalyses the oxidative demethylation of dimethylamine (DMA) to methylamine and formaldehyde:
(CH₃)₂N + H₂O → CH₃NH + CH₂O +2H⁺ + 2e^-[1].

Metabolic Pathway

This enzyme is a complex iron-sulfur flavoprotein that transfers electrons to the soluble flavoprotein known as electron transferring flavoprotein[2]. It couldn't work extracellular isolated.

Figure 1.Pathways for dimethylamine metabolism in bacteria.

Protein Molecular Structures

Dimethylamine dehydrogenase(DMADH) exist as dimers.

Figure 2.Protein molecular structures of dimethylamine dehydrogenase.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 897
Illegal AgeI site found at 1291
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4169016 short</partinfo>
-After expressing, it'll produce dimethylamine dehydrogenase (DMADH). The enzyme DMADH is an iron–sulfur flavoprotein which catalyses the oxidative demethylation of dimethylamine (DMA) to methylamine and formaldehyde: <br>(CH<sub>3</sub>)<sub>2</sub>N + H<sub>2</sub>O → CH<sub>3</sub>NH + CH<sub>2</sub>O +2H<sup>+</sup> + 2e<sup>-</sup>.
+After expressing, it'll produce dimethylamine dehydrogenase (DMADH). The enzyme DMADH is an iron–sulfur flavoprotein which catalyses the oxidative demethylation of dimethylamine (DMA) to methylamine and formaldehyde: <br>(CH<sub>3</sub>)<sub>2</sub>N + H<sub>2</sub>O → CH<sub>3</sub>NH + CH<sub>2</sub>O +2H<sup>+</sup> + 2e<sup>-</sup>[1].
 ==Metabolic Pathway==
 <p>
-This enzyme is a complex iron-sulfur flavoprotein that transfers electrons to the soluble flavoprotein known as electron transferring flavoprotein[1]. It couldn't work extracellular isolated.
+This enzyme is a complex iron-sulfur flavoprotein that transfers electrons to the soluble flavoprotein known as electron transferring flavoprotein[2]. It couldn't work extracellular isolated.
 </p>
 <html>