Difference between revisions of "Part:BBa K4238004"

Revision as of 13:04, 12 October 2022

C120-trucCYC1p+mCherry+ADH1t

When transformed into yeast together with <a href="https://parts.igem.org/Part:BBa_K4238003">BBa_K4238003</a>, this part causes the yeast to produce red fluorescence under blue light. The dimeric EL222 binds to the C120 sequence in the C120-trucCYC1promoter under blue light, allowing transcription of the downstream sequence. The expression can be quantified with the fluorescence of mCherry. A similar part was created in which a kill switch (<a href="https://parts.igem.org/Part:BBa_K4238005">BBa_K4238005</a>) deriving from the human Bax gene is used instead of mCherry.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 104
Illegal SpeI site found at 79
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 86
Illegal SpeI site found at 79
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 104
Illegal SpeI site found at 79
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 104
Illegal SpeI site found at 79
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4238004 short</partinfo>
-When transformed into yeast together with BBa_K4238003, this part causes the yeast to produce red fluorescence under blue light. The dimeric EL222 binds to the C120 sequence in the C120-trucCYC1promoter under blue light, allowing transcription of the downstream sequence. The expression can be quantified with the fluorescence of mCherry. A similar part was created in which a kill switch (BBa_K4238005) deriving from the human Bax gene is used instead of mCherry.
+When transformed into yeast together with <a href="https://parts.igem.org/Part:BBa_K4238003">BBa_K4238003</a>, this part causes the yeast to produce red fluorescence under blue light. The dimeric EL222 binds to the C120 sequence in the C120-trucCYC1promoter under blue light, allowing transcription of the downstream sequence. The expression can be quantified with the fluorescence of mCherry. A similar part was created in which a kill switch (<a href="https://parts.igem.org/Part:BBa_K4238005">BBa_K4238005</a>) deriving from the human Bax gene is used instead of mCherry.
 <!-- Add more about the biology of this part here