Difference between revisions of "Part:BBa K4438808"
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MN4_Target_4(BBa_K4438808) is a single-stranded DNA sequence having 81 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. | MN4_Target_4(BBa_K4438808) is a single-stranded DNA sequence having 81 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
This part along with MN4_trigger_4_phi29(BBa_K4438807) and Aptamer_MN4(BBa_K4438800) can be used to detect different concentrations of quinine [1]. | This part along with MN4_trigger_4_phi29(BBa_K4438807) and Aptamer_MN4(BBa_K4438800) can be used to detect different concentrations of quinine [1]. | ||
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Revision as of 13:02, 12 October 2022
MN4_Target_4
MN4_Target_4(BBa_K4438808) is a single-stranded DNA sequence having 81 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. The 5’ end has few bases complementary to MN4_trigger_4_phi29(BBa_K4438807), the middle region has ssDNA sense T7 promoter sequence and the 5’ end has ssDNA sense broccoli sequence.
Usage and Biology
This part along with MN4_trigger_4_phi29(BBa_K4438807) and Aptamer_MN4(BBa_K4438800) can be used to detect different concentrations of quinine [1].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Reinstein, O., Yoo, M., Han, C., Palmo, T., Beckham, S. A., Wilce, M. C., & Johnson, P. E. (2013). Quinine binding by the cocaine-binding aptamer. Thermodynamic and hydrodynamic analysis of high-affinity binding of an off-target ligand. Biochemistry, 52(48), 8652-8662.